RESEARCH SCIENTIST/ENGINEER 1
Company: University of Washington
Location: Seattle
Posted on: November 8, 2024
Job Description:
If you are using a screen reader and experience any difficulty
accessing our web pages, please call 206-543-2544 or email UWHires
and we will be happy to assist you.Benefits: As a UW employee, you
will enjoy generous benefits and work/life programs. For a complete
description of our benefits for this position, please visit our
website.As a UW employee, you have a unique opportunity to change
lives on our campuses, in our state, and around the world. UW
employees offer their boundless energy, creative problem-solving
skills, and dedication to build stronger minds and a healthier
world.The Department of Medicine - Cardiology has an outstanding
opportunity for a Research Scientist Engineer 1 to join their
team.POSITION PURPOSEField of research this position is engaged in:
The role of plasma extracellular vesicles in heart failure
inflammation.Purpose of the research project(s) this position
supports: Recent studies suggest chronic sterile inflammation
contributes to heart failure (HF) progression; however, a failure
to establish a causal relationship between systemic inflammation
and HF outcomes has essentially halted progress in this field. Our
lab studies how monocytes, a key player in HF inflammation, amplify
the inflammatory signal via extracellular vesicle.Research
Sponsors/Stakeholders: VA, Locke FoundationDUTIES AND
RESPONSIBILITIESConducting Experiments - 80%
- The focus of the Research Scientist Associate position within
the Wang Lab is to perform various molecular and cellular
biological experiments to produce high quality scientific data for
publications, grant applications, and others. More specifically,
the scope of the job includes conducting and designing experiments
using techniques listed below:
- Cell-based assays-culturing and stimulating THP-1 cells with
various pro-inflammatory stimuli and siRNA knockdown screen.
- ELISA of cytokines of culture media and plasma.
- Measuring EV content in culture media and plasma using
FACS.
- Various molecular biological
procedures-immunoprecipitation-Western blot analysis, Protein
purification, RT-qPCR, immunostaining, and cloning.
- Writing: (a) Write a protocol and maintain it updated, (b)
write methodology and result sections of the manuscripts, (c)
generate figures that depict the results of the performed
experiments, and (d) re-format the manuscripts for appropriate
journal styles.
- Quantification of scanned or acquired images such as electron
microscopic and immunohistochemistry images using image-analysis
software.The Research Scientist will report to and work closely
with the Principal Investigator, Dr. Dennis Wang, who will mentor
him/her for his/her professional goals and
development.Operations/Administration - 20%
- Independently and collaboratively support lab management and
operation as part of the research activity, such as identification
and procurement of laboratory supply and service.
- Support and participate in regulatory and certification
processes and laboratory compliance.
- Support and participate in laboratory orientation of new lab
personnel.
- Collaborate with and support the PI/supervisor with multiple
laboratory experiments, as delegated by the PI/supervisor.
- Prepare, optimize and execute experiment protocols; analyze the
data and generate and present summary and reports for research
projects and laboratory activities per instruction.MINIMUM
REQUIREMENTS
- Bachelor's Degree in Biochemistry, Molecular Biology, Cell
Biology, Biotechnology and 1 year of laboratory experience.
Equivalent education/experience will substitute for all minimum
qualifications except when there are legal requirements, such as a
license/certification/registration.ADDITIONAL REQUIREMENTS
- Excellent communication skills and good people skills.
- Self-motivator with positive attitude.
- Ability to produce the data in a reproducible fashion.
- Ability to adhere to the established protocols.
- Ability to improve and trouble-shoot a protocol.
- Good hands in basic experiments.
- Experience and significant understanding in molecular biology
and/or biotechnology.DESIRED QUALIFICATIONS
- PhD in Biochemistry, Molecular Biology, Cell Biology,
Biotechnology.
- Some real wet lab experience and superior ability to learn the
new techniques and protocols.Application Process: The application
process may include completion of a variety of online assessments
to obtain additional information that will be used in the
evaluation process. These assessments may include Work
Authorization, Cover Letter and/or others. Any assessments that you
need to complete will appear on your screen as soon as you select
"Apply to this position". Once you begin an assessment, it must be
completed at that time; if you do not complete the assessment, you
will be prompted to do so the next time you access your "My Jobs"
page. If you select to take it later, it will appear on your "My
Jobs" page to take when you are access ready.
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Keywords: University of Washington, Everett , RESEARCH SCIENTIST/ENGINEER 1, Engineering , Seattle, Washington
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